![]() ![]() Several studies ( 12, 22, 29) indicate an abnormal immune reactivity to intestinal flora as the cause for the inflammation, probably caused by impairment of the mucosal barrier function. Inflammatory bowel disease (IBD) in its more common forms, Crohn's disease (CD) and ulcerative colitis, is characterized by presenting alternative phases of activity and quiescence and by its chronicity ( 11). However, as in IBD, this reaction seems unable to prevent a return to relapse. This phase is followed by a reactive one characterized by hypermotility associated with a decrease in both bacterial growth and leukocytes. During the high-leukocyte stage, motor activity decrease is related to bacterial translocation. We obtained a model of IBD characterized by a chronic cyclic oscillation of intestinal motility, flora, and inflammatory blood parameters. Bacterial overgrowth accompanied by bacterial translocation was found in the group with high leukocytes, whereas no differences were observed between the control and indomethacin groups during the lowest leukocyte phase. ![]() In contrast, animals with low leukocyte levels presented hypermotility. Rats with high leukocyte levels showed a decrease of motor activity. Controls were obtained in age-matched rats. ![]() Treated rats were then selected either during their highest or lowest blood leukocyte values for motor activity and microbiological evaluation. In treated rats, a cyclic oscillation of blood leukocytes and TNF concomitant with an inverse correlation of fecal output was observed. Blood leukocytes, TNF, and fecal parameters were monitored for 90 days after treatment. For this purpose, two groups of Sprague-Dawley rats were used: a treated group that received two subcutaneous injections of indomethacin (7.5 mg/kg) 48 h apart and a control group that received saline. The aims of this study were: 1) to obtain an experimental model reproducing the characteristics of chronicity and spontaneous relapses found in inflammatory bowel disease (IBD) and 2) to correlate these changes with intestinal motility and bacteria translocation. ![]()
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